FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n01_t3rr1r201.351000000589c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n01_t3rr1r201.351000000589c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9073255
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2360032.6010841754144463TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC122120.13459337360186616TruSeq Adapter, Index 7 (100% over 51bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG109940.121169304731323TruSeq Adapter, Index 7 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC304700.037.8396245
CATCTCG319050.034.74165740
TCGTATG333700.034.45042844
CTCGTAT333600.034.35957743
ATCTCGT333800.033.3143541
TCTCGTA336200.033.0966142
TCATCTC346450.031.82848539
GATCATC359900.030.6694237
ATCATCT374700.029.4403438
GTGACTG35000.028.103781
GACTGGA36750.026.4529953
TGACTGG37450.025.89862
AGATCAT430100.025.72625436
GGAGTTC41950.023.2762417
CTGGAGT46450.021.1225725
GTATGCC43500.019.34605845
TCCCTAC34800.018.9427689
CCCTACA34550.018.8193610
ACTGGAG52800.018.6675014
GAGTTCA54350.018.1313298
Produced by FastQC (version 0.11.2)