FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n01_t3r1r201.3510000005893f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n01_t3r1r201.3510000005893f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12849002
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC2788552.1702463739985407TruSeq Adapter, Index 22 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC170320.13255504201804935TruSeq Adapter, Index 22 (98% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC145440.11319167045035872TruSeq Adapter, Index 14 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC384200.036.65501445
CTCGTAT401900.034.436243
TCGTATG414600.033.78837644
ATCTCGT395750.033.4135241
TCTCGTA398500.033.23940342
GATCTCG410250.031.96928640
ACGATCT409300.031.9537938
TACGATC416300.031.51377537
GTACGAT493400.026.79900436
CGATCTC512950.025.51035939
GTGACTG38750.021.7247491
GACTGGA40200.020.7125783
TGACTGG41100.020.2042642
GTATGCC61700.020.0204145
CGTACGA716100.019.31951935
GGAGTTC44750.019.1051737
CCGTACG761600.018.16532134
ACCGTAC824800.016.82523733
CACCGTA831150.016.71564332
TCACCGT847450.016.44988331
Produced by FastQC (version 0.11.2)