FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n01_t2rr1r201p.35100000058a14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n01_t2rr1r201p.35100000058a14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16785085
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC287056417.10187347874616TruSeq Adapter, Index 16 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC1764121.0510045078711248TruSeq Adapter, Index 16 (98% over 51bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCA1128430.6722813736123469TruSeq Adapter, Index 16 (97% over 39bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC925610.5514479074726163TruSeq Adapter, Index 16 (100% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTT786570.4686124615990923TruSeq Adapter, Index 16 (98% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC613430.3654613604875996TruSeq Adapter, Index 14 (97% over 38bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG592050.35272386169030423TruSeq Adapter, Index 16 (97% over 49bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC560830.334124015457771TruSeq Adapter, Index 16 (100% over 39bp)
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCAT529140.3152441587278229TruSeq Adapter, Index 16 (97% over 41bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG465340.27723422312130086TruSeq Adapter, Index 16 (97% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG340020.2025727007042264TruSeq Adapter, Index 16 (97% over 49bp)
CACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC313340.18667763672331716Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCAACTCGTATGC299380.1783607291830813TruSeq Adapter, Index 16 (97% over 39bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC275210.16396104041177034TruSeq Adapter, Index 16 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCACCTCGTATGC270710.1612800888407774TruSeq Adapter, Index 16 (97% over 39bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCA252400.150371594781915TruSeq Adapter, Index 16 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCG249300.1485247170330088TruSeq Adapter, Index 16 (98% over 51bp)
AAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATC199510.11886147731751134TruSeq Adapter, Index 16 (97% over 42bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCAT190430.11345191281426337TruSeq Adapter, Index 16 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGCG10050.040.52491840
CGTATGC3581900.038.94384845
ATCGCGT10600.038.63448741
CCGTCTT107850.038.5142345
GCCGTCT107200.038.20189744
TCGTATG3752750.036.98847644
CTCGTAT3759550.036.71149443
TCTCGTA3673550.036.605642
CATCTCG3677200.036.4543340
GTGACTG25900.036.0611421
ATCTCGT3742900.035.90341641
CGCGTAT12050.034.91908643
CCATCTC3847000.034.7677439
GCGTATG12700.034.54929744
CAGCTCG21600.034.37714840
TCCATCT3896600.034.29280538
AGCTCGT21700.034.21872341
ACACTCT159600.033.998161
GTCCATC3981850.033.6727537
ACCTCGT39250.033.5944541
Produced by FastQC (version 0.11.2)