FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n01_t2rr1r201.351000000589a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n01_t2rr1r201.351000000589a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10092773
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC4833804.789367599964846TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC264990.2625542058659201TruSeq Adapter, Index 11 (100% over 51bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA169910.16834818339816024TruSeq Adapter, Index 11 (100% over 40bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA131840.13062812370792448TruSeq Adapter, Index 11 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC119130.11803495431830281TruSeq Adapter, Index 14 (97% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTT109310.10830521998265491TruSeq Adapter, Index 11 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC605850.039.02228545
CATCTCG612400.037.15415640
TCGTATG635850.036.9656844
CTCGTAT634650.036.9292143
TCTCGTA627600.036.40829542
ATCTCGT633500.036.05873541
ACATCTC642900.035.27339639
TACATCT661300.034.24346538
CTACATC676000.033.58868837
GCTACAT776000.029.29504236
GTGACTG27100.023.4209061
GCCGTCT27250.023.03780444
GTATGCC78750.022.45799645
GACTGGA29050.021.6895083
TGCCGTC29950.021.26145443
TGACTGG29650.021.2505992
GGCTACA1121650.020.80551135
CCGTCTT32400.020.13965645
ATGCCGT32500.019.73171642
CGGCTAC1190700.019.59897634
Produced by FastQC (version 0.11.2)