FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n01_t2r1r201.35100000058915.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n01_t2r1r201.35100000058915.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12786322
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC2122451.6599378617244271TruSeq Adapter, Index 14 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC288140.22535018279689814TruSeq Adapter, Index 14 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC291650.035.81228645
CATCTCG283500.035.26270340
CTCGTAT295650.034.78783443
ATCTCGT299700.033.5217841
TCGTATG312350.033.25924744
TCTCGTA305450.032.9938742
CCATCTC328000.030.43552239
TCCATCT341300.029.26926438
TTCCATC359050.027.7909837
GTTCCAT372550.026.85628936
AGTTCCA525850.019.42086435
GACTGGA32200.018.729623
CAGTTCC551350.018.46959734
TGACTGG32100.018.437522
GTATGCC47600.018.1515345
ACAGTTC580650.017.59185633
TCACAGT583900.017.53247331
CACAGTT588000.017.44848632
GTCACAG676800.017.36320730
AGTCACA684100.017.16805629
Produced by FastQC (version 0.11.2)