FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n01_t1rr1r201p.351000000589f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n01_t1rr1r201p.351000000589f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8518060
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC4785745.6183450222233695TruSeq Adapter, Index 18 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCC375980.44139158446876403TruSeq Adapter, Index 18 (100% over 51bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCCGTCTT109060.1280338480827794TruSeq Adapter, Index 18 (100% over 51bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCA108200.12702422852151782TruSeq Adapter, Index 18 (100% over 40bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG105330.12365491672986571TruSeq Adapter, Index 18 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC616350.038.0682445
CATCTCG621650.036.1700740
TCGTATG645950.036.1644244
CTCGTAT646700.036.0215843
TCTCGTA631900.035.74715842
ATCTCGT632700.035.63438841
GCATCTC647950.034.57449339
CGCATCT647700.034.47300338
CCGCATC670350.033.42904737
TCCGCAT748700.029.9427836
GTATGCC92100.027.11768345
GTCCGCA1014350.022.8046835
CGTCCGC1070500.021.61903634
GCCGTCT27450.021.47624444
TGCCGTC29550.020.4830343
ACGTCCG1131800.020.46799533
CCGTCTT29300.020.42693745
CACGTCC1145650.020.25001132
TCACGTC1152550.020.16001531
GTCACGT1168700.019.91993130
Produced by FastQC (version 0.11.2)