FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n01_t1rr1r201.3510000005898f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n01_t1rr1r201.3510000005898f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11362488
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC8327787.329187058327366TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC510220.44903897808296916TruSeq Adapter, Index 22 (97% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCA306000.26930721510993016TruSeq Adapter, Index 22 (97% over 37bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAG271080.23857450938562047TruSeq Adapter, Index 22 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTT268960.23670872083649286RNA PCR Primer, Index 24 (97% over 37bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG222020.19539734607420486TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC165210.14539949349121423TruSeq Adapter, Index 14 (97% over 38bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG160250.14103425235740624TruSeq Adapter, Index 22 (97% over 37bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGC157290.13842918910013371TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAACTCGTATGC128200.11282740188592498TruSeq Adapter, Index 22 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG120550.10609472150817673TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1075350.038.5402845
TCGTATG1139500.036.16997544
CTCGTAT1146650.035.8011843
CATCTCG1107550.035.64309340
TCTCGTA1117850.035.4876342
ATCTCGT1135850.034.8996541
GCATCTC1197400.032.82874339
AGCATCT1220300.032.1512738
TAGCATC1257050.031.32752837
GCCGTCT50850.029.91327344
CCGTCTT52800.029.19142745
TGCCGTC53050.028.80000743
GTGACTG42800.027.0283371
ATGCCGT57200.026.59248442
GTAGCAT1560150.025.2716236
TGACTGG47400.024.3534562
CACCTCG15650.023.86733240
GACTGGA48650.023.820233
GTATGCC148750.023.80928645
ACTCGTA32250.022.6059942
Produced by FastQC (version 0.11.2)