FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n01_t1r1r201.351000000588fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n01_t1r1r201.351000000588fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8572976
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC2238202.6107620037662533TruSeq Adapter, Index 13 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC132780.1548820386292928TruSeq Adapter, Index 13 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC293550.037.15162345
CTCGTAT302900.035.55190343
AATCTCG290900.035.37869640
TCGTATG308800.035.07665644
TCTCGTA294850.035.07262442
ATCTCGT294900.034.99038341
AAATCTC327850.031.37691939
CAAATCT329150.031.19056338
TCAAATC348100.029.58937537
GTCAAAT382850.027.05041136
GTATGCC47000.021.59064145
AGTCAAA548050.019.68099235
GTGACTG24900.019.253471
CAGTCAA573950.018.8242334
GACTGGA25600.018.6354983
TGACTGG26100.018.2784962
CACAGTC611850.017.70967732
ACAGTCA611450.017.67342633
ACTCGTA17400.017.58648342
TCACAGT621350.017.48960331
Produced by FastQC (version 0.11.2)