FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n01_t0rr1r201p.351000000589ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n01_t0rr1r201p.351000000589ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14080
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGATCGGAAGAGCAC3152.237215909090909TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGATCGGAAGAGCAC2151.5269886363636365TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC1831.299715909090909TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGATCGGAAGAGCAC1310.9303977272727273TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGATCGGAAGAGCAC1200.8522727272727272TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGATCGGAAGAGCAC980.6960227272727273TruSeq Adapter, Index 3 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACCCAGATCGGAAGAGCACACGTCT840.5965909090909092TruSeq Adapter, Index 5 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATCGGAAGAGCAC610.43323863636363635TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGATCGGAAGAGCAC480.3409090909090909TruSeq Adapter, Index 2 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTAGATCGGAAGAGCACACGTCT410.2911931818181818TruSeq Adapter, Index 3 (96% over 32bp)
GAAGAGCACACGTCTGAACTCCAGTCACTTAGATCGGAAGAGCACACGTCT390.27698863636363635TruSeq Adapter, Index 3 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAAGATCGGAAGAGCAC370.2627840909090909TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGATCGGAAGAGCAC360.2556818181818182TruSeq Adapter, Index 5 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACATAGATCGGAAGAGCACACGTCT340.2414772727272727TruSeq Adapter, Index 3 (96% over 32bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGTGACTGGAGTTCAGAC280.19886363636363635Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACTCAGATCGGAAGAGCACACGTCT260.1846590909090909TruSeq Adapter, Index 3 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATCGGAAGAGCAC240.17045454545454544TruSeq Adapter, Index 25 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTACGAGTGGATCTCGTATGCC220.15625Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGATCGGAAGAGCACA190.13494318181818182TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGATCGGAAGAGCACA190.13494318181818182TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGATCGGAAGAGCAC160.11363636363636363TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAGATCGGAAGAGCAC150.1065340909090909TruSeq Adapter, Index 7 (97% over 35bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGAGATCGGAAGAGCACACGTCT150.1065340909090909TruSeq Adapter, Index 5 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGAG750.036.1114274
TGACTGG701.6370905E-1135.466582
GTGACTG701.6370905E-1135.466581
GACTGGA701.6370905E-1135.466583
CTGGAGT801.8189894E-1233.8544655
TAAGATC300.00498965629.98576517
TGGAGTT1051.8189894E-1227.9433676
GGAGTTC1157.2759576E-1225.4227147
CTTAGAT602.3109237E-422.48932534
AGTCACT902.8491922E-722.48932329
GAGTTCA1356.184564E-1121.6563878
GTCACTT653.9710628E-420.75937530
ACTTAGA653.9710628E-420.75937533
CACTTAG653.9710628E-420.75937532
TCACTTA653.9710628E-420.75937531
TTAGATC706.546178E-419.27656235
CGATCTC600.006220090718.74110429
CAGTCAC3850.016.9400128
GTCACCC850.002407748315.87481740
CACCCAG850.002407748315.87481742
Produced by FastQC (version 0.11.2)