FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n01_t0rr1r201.35100000058959.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n01_t0rr1r201.35100000058959.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10513108
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC121745511.580352831912313TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC497600.47331388586515044TruSeq Adapter, Index 7 (97% over 36bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA402590.38294099137952353TruSeq Adapter, Index 7 (97% over 36bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG401960.38234173947418787TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCCGTCTT385460.36664704671539566RNA PCR Primer, Index 23 (97% over 37bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG302950.2881640709864295TruSeq Adapter, Index 7 (97% over 36bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTG266480.2534740440219962TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAACTCGTATGC258910.24627350922296243TruSeq Adapter, Index 7 (97% over 36bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG220910.2101281562027138TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC184940.17591372598854688TruSeq Adapter, Index 14 (97% over 38bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGG173020.1645754994621952TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGACCTCGTATGC140430.13357610327982933TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAGCTCGTATGC133830.12729822617631245TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1536750.039.4273845
TCGTATG1648150.036.6177344
GCCGTCT60800.036.1579844
CTCGTAT1654950.036.05121643
TCTCGTA1586950.035.8874142
CCGTCTT62450.035.77914445
ATCTCGT1610700.035.31789841
GGATCTC1630050.034.73813639
TGGATCT1652400.034.19529738
GATCTCG1677900.033.8108840
GTGGATC1702200.033.29384237
GAGCTCG22100.031.8689240
GGAGCTC23050.030.55268939
TGCCGTC72850.030.45513543
ATGCCGT76000.029.22245642
ACACTCT68500.029.1754931
AGCTCGT24950.028.8598941
GACCTCG24250.027.46598840
ACTCGTA50100.027.44205742
ACCTCGT24450.027.3333541
Produced by FastQC (version 0.11.2)