FastQCFastQC Report
Wed 8 Jun 2016
HJ5TCBCXX_l01n01_t0r1r201.351000000588ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5TCBCXX_l01n01_t0r1r201.351000000588ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10187786
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC830850.8155353871783331TruSeq Adapter, Index 21 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC128900.031.40284745
CTCGTAT129650.030.9266143
GATCTCG128400.030.10600940
ATCTCGT131650.029.6362541
TCGATCT132300.029.1486438
TCGTATG142700.028.25603344
TCTCGTA141000.027.76675842
TTCGATC150350.025.60423337
CGATCTC157650.024.4616839
TTTCGAT196750.019.78310436
GTGACTG21150.016.921611
GACTGGA21650.016.6308573
TGACTGG22350.016.2107472
GTTTCGA251050.015.88986235
CGTTTCG250650.015.879312534
ACGTTTC277600.014.3944533
CACGTTT279550.014.31013832
AGTCACG282850.014.2784129
GTCACGT281200.014.26617730
TCACGTT292100.013.72611831
Produced by FastQC (version 0.11.2)