FastQCFastQC Report
Wed 8 Jan 2025
HJ5N3DRX5_n02_wy_Petrov_10X.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5N3DRX5_n02_wy_Petrov_10X.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833471797
Sequences flagged as poor quality0
Sequence length90
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG81549100.9784266281538019Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGACCTCAAATCAGGTA16863700.2023307814457458Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGGAG10810200.12970084937379112Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGGA8717030.1045869822035502Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAATAAGCGGAGGAAAAG8560270.10270617471175211Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATATCAATAAGCGGAGGAAA8406210.10085776183738103Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)