FastQCFastQC Report
Wed 8 Jan 2025
HJ5N3DRX5_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5N3DRX5_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43163365
Sequences flagged as poor quality0
Sequence length90
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG3237480.7500527356937996Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG807760.18714018242090255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGACCTCAAATCAGGTA668080.15477940610052066Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGGAG434630.1006941882311539Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)