FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32841063
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18013965.485193947589333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC90950.034.20983513
CGTCTGA87950.034.10466416
ACGTCTG90100.033.5606615
CACGTCT96350.031.85551514
CACACGT102550.031.22655912
GCACACG107850.030.1136811
ATCGGAA137950.029.8619082
GATCGGA141100.029.5374241
TCGGAAG139800.028.487153
CGGAAGA139950.027.8571324
GAACTCC122900.023.57366821
AGCACAC159300.021.4192210
AGAGCAC168500.021.1627398
CCGATCA10100.020.81183631
CTGAACT141300.020.75894519
TCCGATC9800.020.7375130
AACTCCA151300.019.19800222
CTCCAGT151050.017.68652324
TGAACTC174500.017.25221320
CCAGTCA123350.016.42002926
Produced by FastQC (version 0.11.5)