FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG077.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG077.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24270755
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21500.016.27927444
TCGTATG21800.015.57375143
CTCGTAT21300.014.95351742
TATGCCG24600.014.36992246
ATGCCGT25900.014.18919247
TCTCGTA21500.013.83763941
TGCCGTC31550.011.20427848
ATCTCGT30700.010.14689440
GCCGTCT37650.09.48189749
CCGTCTT38250.09.33312450
CGTCTTC45200.07.9755551
CATCTCG39550.07.78782139
GTATGCC58900.06.59595445
ACGTCTG192550.05.762271470
CGTCTGA169050.05.38314670
CACGTCT213900.05.203495569
ACACGTC212950.05.14458168
Produced by FastQC (version 0.11.5)