FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG076.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG076.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23821606
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG20300.017.93159143
CTCGTAT20000.017.50076342
TCTCGTA20600.017.16097841
CGTATGC22100.016.94597844
TATGCCG24500.014.57142246
ATGCCGT25400.014.46831547
AATCTCG25750.013.59279739
TGCCGTC30850.011.68532548
ATCTCGT31100.011.36707940
CCGTCTT34550.010.5352350
GCCGTCT36600.010.51889949
CGTCTTC45700.08.2712151
GTATGCC54200.07.29712145
CGTCTGA92750.05.01863416
Produced by FastQC (version 0.11.5)