FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG075.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG075.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20106625
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG17300.015.37653943
CGTATGC18000.014.77834244
CTCGTAT17550.014.75872942
TCTCGTA18850.013.18382341
TATGCCG20000.012.94985146
ACGGCTA23200.012.068706532
ATGCCGT22050.011.74583747
ATCTCGT25550.010.54855940
TGCCGTC27750.09.71145548
CGGCTAC29200.09.46899833
CCGTCTT31900.08.33827650
CATCTCG32950.08.28576339
TCACGGC37150.07.81919130
GCCGTCT33500.07.626625549
CGTCTTC40650.06.629607751
GTATGCC45700.06.50993445
GTCACGG42300.06.453521329
CACGGCT47150.06.235161331
AGTCACG44900.06.157767328