FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG074.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG074.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23762283
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG21500.021.97804843
CGTATGC24300.021.46195244
CTCGTAT22000.021.31962642
TCTCGTA23900.019.33189441
ATGCCGT27250.019.00890447
TATGCCG27300.018.97452746
TATCTCG28500.016.33437239
ATCTCGT32300.014.95460640
TGCCGTC35750.014.78281948
CCGTCTT41900.011.86118150
GCCGTCT44450.011.49572949
CGTCTTC47050.010.48858151
GTATGCC58250.09.79430445
TATCGCG4201.01042584E-49.16724939
CGCGTAT3550.00207545218.8738442
GCTTATC63100.08.37598636
TAGCTTA86200.06.69952534
AGCTTAT88950.06.6499635
ACTAGCT94800.06.16549832
GTCACTA95450.06.086574629
Produced by FastQC (version 0.11.5)