FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG073.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG073.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22693935
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC5063102.231036618374028TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG639950.062.01221543
CTCGTAT621550.061.8551842
TCTCGTA608500.061.81272541
CGTATGC643800.061.77633744
ATGCCGT644100.061.47966847
TATGCCG646200.061.3941646
CGATCAG648350.061.3295833
TGCCGTC646450.061.217648
ACGATCA652200.061.06882532
CCGTCTT646350.060.9881450
GCCGTCT647600.060.93541349
GATCTCG608300.060.68196539
CACGATC655900.060.66430331
GTCACGA658050.060.5663529
ATCTCGT610300.060.5005840
CGTCTTC652300.060.443151
TCACGAT659800.060.40040230
CGTCTGA683150.059.3963916
GTATGCC670150.059.22654345
AGTCACG673500.059.20813828
Produced by FastQC (version 0.11.5)