FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG072.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG072.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25359803
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC355790.14029683117017905TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT53200.049.87042242
TCTCGTA53150.049.7857341
TCGTATG54700.049.39820543
CGTATGC57600.049.2806244
ATGCCGT60100.047.17113547
AATCTCG61700.044.41831639
TATGCCG65100.044.08605246
TGCCGTC64950.042.89380348
TCGCGTA5000.042.0017741
GCCGTCT63350.041.49084549
ATCTCGT66950.040.20333540
CCGTCTT67050.039.670950
CGTCTTC72150.036.09093551
CGCGTAT6000.035.58476342
GTATGCC92200.031.46996345
ATCGCGT7450.030.06837840
CGTCTGA121250.027.59459116
ACGTCTG129850.025.90186115
ACACGTC138400.024.2516613
CACGTCT148650.022.6731914
Produced by FastQC (version 0.11.5)