FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18816494
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC224000.11904449362351986TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT37200.048.3621642
TCGTATG39550.046.5501743
TCTCGTA37250.046.23004541
CGTATGC42550.044.50131244
ATGCCGT42450.044.4399447
TATGCCG45450.041.35281846
TGCCGTC45350.041.21181548
GCCGTCT45550.040.03209349
CCGTCTT47300.038.8469750
CGTCTTC50700.036.4492351
ATCTCGT49050.035.25116340
CATCTCG51050.033.18422339
GTATGCC67700.027.81381845
CGTCTGA78000.025.30675716
ACGTCTG81050.024.26813915
ACACGTC86650.022.66025413
GCTTGAA89600.021.015859
CACGTCT96800.020.57264314
TCGCGTA2051.4588295E-920.48862841
TTAGGCA95400.020.43485334
Produced by FastQC (version 0.11.5)