FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG070.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG070.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20316473
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC393280.19357690677904574TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG62550.053.21516443
CTCGTAT59800.052.73661442
CGTATGC63000.052.61205344
TCTCGTA59150.051.8960841
ATGCCGT64550.051.13068847
TATGCCG67050.049.22485746
CGATCTC63250.047.6462938
TGCCGTC70250.046.78191448
CCGTCTT71850.046.03241350
GCCGTCT71850.045.9349949
TCACGAT68750.045.9201435
ACGATCT65600.045.67255837
CACGATC67300.044.46642336
CGTCTTC75150.044.10428251
GATCTCG69550.043.53168539
ATCACGA75350.043.19801334
ATCTCGT70900.042.75227740
CATCACG80500.041.86907633
GTATGCC91950.036.31350745
CGTCTGA109200.031.44101316
Produced by FastQC (version 0.11.5)