FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG069.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG069.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23332657
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1042680.4468758101574115TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG136450.059.640543
CTCGTAT131350.059.5318842
CGTATGC138650.059.17267244
TCTCGTA131500.058.8251541
ATGCCGT141300.057.8872947
TATGCCG142300.057.43241546
AATCTCG134450.057.16948739
TGCCGTC145950.055.7543948
GCCGTCT149250.054.0525149
CCGTCTT149350.054.0395250
ATCTCGT145300.052.9009240
CGTCTTC153200.052.49893651
GTATGCC169850.048.36425445
GTAATCT180500.042.73937
CGTCTGA200450.042.54962516
GCTTGAA203700.040.103459
ACGTCTG213250.040.07779315
ACACGTC220900.038.76990513
TGTAATC204000.038.43265536
CACGTCT229650.037.27683614
Produced by FastQC (version 0.11.5)