FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG068.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG068.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24574485
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC580300.2361392314020009TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG79450.056.3014743
CTCGTAT78300.056.18998742
TCTCGTA77850.054.85137641
CGTATGC84900.054.6652844
ATGCCGT87250.052.99180247
TATGCCG89950.051.906946
TGCCGTC90250.050.99688348
GCCGTCT91900.049.0906749
CCGTCTT95950.047.60201650
CGTCTTC100450.045.05168551
ATCTCGT95300.044.6607640
CATCTCG97850.043.78296339
GTATGCC117350.040.115545
TCGCGTA4700.038.72503341
CGCGTAT5550.035.94738442
CGTCTGA142150.034.96126616
GATCATC129100.034.94632736
ACGTCTG147750.033.61248415
ACACGTC157850.031.59540413
GCTTGAA155050.029.79706459
Produced by FastQC (version 0.11.5)