FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG067.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG067.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22169418
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1816020.8191554690339639TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA227950.062.2489361
ATCGGAA231050.061.4741972
CGTATGC220600.061.41730544
CTCGTAT218100.061.36830542
TCGTATG220450.061.33249743
TCTCGTA218750.061.0260841
ATGCCGT222000.060.8077547
CGCCAAT225850.060.7167633
TATGCCG222750.060.6665546
TATCTCG220900.060.3688839
TGCCGTC225200.059.80356248
CGTCTGA233050.059.71010616
ATCTCGT225150.059.16715240
GCCGTCT226900.059.12384849
CCGTCTT227250.059.03265850
ACGTCTG236550.058.96005215
ACGCCAA233300.058.91237332
GTCACGC233900.058.67001329
TCGGAAG243500.058.347023
ACACGTC240350.058.3352513
Produced by FastQC (version 0.11.5)