FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG066.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG066.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18653685
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC3356781.7995264742596435TruSeq Adapter, Index 15 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA423450.066.161521
ATCGGAA426650.065.558992
CTCGTAT391750.064.2490742
TCGTATG410550.064.2305243
CGTATGC412500.064.0784244
CGTCTGA425050.063.96079316
TCTCGTA386000.063.95497541
TCGGAAG436000.063.834353
AATCTCG383850.063.5923139
TATGCCG415800.063.4506546
ATGCCGT415050.063.44725447
ACGTCTG429150.063.36638315
ATCTCGT386700.063.27784740
ACACGTC431150.063.18050813
CCGTCTT417000.062.94827750
TGCCGTC419150.062.7927248
GCCGTCT420800.062.4047849
CGGAAGA445350.062.1877984
CGTCTTC424050.061.9679351
CACGTCT441050.061.75984614
Produced by FastQC (version 0.11.5)