FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20613469
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1810490.8783043746785173TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA252050.061.0062681
CTCGTAT223650.060.89413542
TCGTATG236200.060.66605843
TCTCGTA221950.060.24088741
ATGCCGT238400.060.03063247
ATCGGAA256850.059.9615822
CGTATGC240150.059.79910344
TATGCCG239600.059.78869646
CGTCTGA251550.059.22808516
ACACGTC255400.058.62544613
ACGTCTG254800.058.5825115
AATCTCG226300.058.29369439
TGCCGTC245600.058.0992148
CCGTCTT247150.057.4799450
ATCTCGT231150.057.29811540
GCCGTCT252200.056.30121249
TCGGAAG274250.056.056263
CGTCTTC255950.055.5178951
CACGTCT270000.055.4142914
GTATGCC259150.055.41403245
Produced by FastQC (version 0.11.5)