FastQCFastQC Report
Wed 13 Nov 2019
HJ5M5BGXC_n01_GG064.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5M5BGXC_n01_GG064.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23374914
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1231030.526645787873273TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT153200.059.6536742
TCGTATG155950.059.3195843
CGATGTA161550.058.9512534
TCTCGTA154700.058.1703741
TATGCCG162750.057.892846
CGTATGC163600.057.48676744
TATCTCG157350.057.2795539
ATGCCGT163800.056.83744447
CACCGAT170800.056.39135431
ACCGATG172900.055.60641532
CCGATGT172350.055.50027533
ATCTCGT165000.054.68761440
CGTCTGA184800.053.3113916
GATCGGA188150.053.1670381
ACGTCTG186250.053.0094315
TGCCGTC175500.052.84862548
ACACGTC188500.052.26652513
GTATGCC182350.051.9394345
CCGTCTT176400.051.82458550
ATCGGAA194500.051.50292
Produced by FastQC (version 0.11.5)