FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91292049
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG136050.063.587165
CTATACG98100.063.1384246
TATAGGG241400.062.3187147
GTATAGG246450.062.2204326
TATACGG141950.062.1724557
GCTATAC115300.061.7667275
CGTATAA125300.061.613225
GGTATAC127050.061.0950975
CTTATAG130900.061.062695
GGTATAG233350.060.8138545
GTATAGC154000.060.6708456
AGTATAG180200.059.9905175
TTATAGT236900.059.8836366
TATAAGG187550.059.72517
CTATAGG134750.059.677526
GTATAGT249050.059.4070436
TATAGGC169550.059.130997
CCTATAC92450.059.0144925
GCGTATA163350.058.915784
GTATACG134700.058.608556
Produced by FastQC (version 0.11.5)