FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS90mS3-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS90mS3-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5208225
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG68450.065.851256
GGTATAG116650.065.194225
TTATAGG119050.065.112736
CGTATAA61400.065.0926365
CTTATAG63500.065.034295
CTATACG48300.064.993056
CGTATAG66950.064.767155
CCTATAC45000.064.5505145
GGTATAC62450.064.0542455
GTATAGG128300.063.9643176
GTATAGC78950.063.609416
GCTATAC57000.063.5476575
TATACGG70550.063.3460587
TATAGGG130250.063.3028567
CTATAAC47950.063.058956
TATAGGC91150.062.8518077
GGTATAA89900.062.4812135
TTATAGC83800.062.4336556
GTTATAC72700.062.051545
TATAGTG136050.062.018957
Produced by FastQC (version 0.11.5)