FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS90mS3-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS90mS3-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8320561
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG115450.066.473126
CCTATAC68550.066.213165
GTATAGG216850.065.309316
TTATAGG200250.065.043696
GGTATAG193600.064.911535
CTATACG81100.064.897556
CTTATAG108400.064.825555
CGTATAA96850.064.8238145
CGTATAG118600.064.7976765
TATAGGC147700.064.01867
AGTATAG150600.063.740085
GGTATAC110350.063.7117275
TATACGG123950.063.213367
GCTATAC96950.063.060425
TATAGGG218600.062.8974767
GTATAGC130550.062.8321726
TTATAGT205850.062.645226
TATAGGT217800.062.550087
GGTATAA150400.062.545095
GTATAAG155000.062.3581666
Produced by FastQC (version 0.11.5)