FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS90mS2-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS90mS2-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4020795
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG49900.065.645586
TTATAGG91800.064.54236
TATACGG58550.064.435837
CCTATAC30950.064.229425
CTATACG38350.064.06226
CTTATAG51000.063.888975
GGTATAG93800.063.8774075
CGTATAG54750.063.4762845
GTATAGG98300.063.4074946
TATAGGG97650.062.9702037
CGTATAA44150.062.7828255
GTTATAA68400.062.4749535
GCTATAC43450.062.344415
CTATAAC35850.062.2818766
GTTATAG93800.062.1983875
TATAGTG109900.062.0335777
GTATAGC63500.062.0024646
GTATAAG70800.061.9861156
GGTATAC53300.061.985655
TTATAGT94700.061.6049736
Produced by FastQC (version 0.11.5)