FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS90mS2-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS90mS2-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7186707
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC61350.066.284535
CTATAGG102550.065.895876
CTATACG71950.065.856636
GTATAGG179300.065.013866
TTATAGG170850.064.9315646
CGTATAG102200.064.547755
GGTATAG163350.064.529235
CGTATAA85450.064.463415
CTTATAG89200.064.460455
TATACGG104200.064.3822257
TATAGGG187850.063.750037
GCTATAC83750.063.6826025
GTATAGC114550.063.453126
GGTATAC96250.063.265825
CTATAAC69950.062.8367166
AGTATAG129900.062.718335
TTATAGC121750.062.696
TATAGGC128450.062.6893967
TATAGGT189200.062.5367977
CTTATAC93350.062.494525
Produced by FastQC (version 0.11.5)