FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS90mS1-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS90mS1-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4858326
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG69850.066.381466
CCTATAC42000.065.4073645
CGTATAA54550.065.242795
CTTATAG61550.064.8729865
TTATAGG108500.064.73146
CGTATAG64750.064.585415
GTATAGG120900.064.344366
GGTATAG114900.064.325065
CTATACG47300.064.2918556
TATACGG68150.063.466647
TATAGGG123900.063.378867
GGTATAC63750.063.2929575
GCTATAC55100.063.2577935
GGTATAA84800.062.9745375
GTTATAA78300.062.7050065
GTATAGC74450.062.562016
TTATAGC78300.062.5249446
CTATAAC46050.062.46546
TATAGTG128500.062.362757
AGTATAG90500.062.140345
Produced by FastQC (version 0.11.5)