FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS90mS1-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS90mS1-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7100767
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG94450.065.618326
CTATACG69150.065.334496
GTATAGG183950.065.29146
CCTATAC56800.065.124025
CGTATAG100200.065.101765
CGTATAA89900.065.086535
TTATAGG174150.065.0068746
CTTATAG91300.064.893435
GGTATAG163100.064.1981055
AGTATAG129250.064.088815
GTATAGC109750.063.5812536
TATACGG108450.063.4726037
GCTATAC80350.063.4147155
TATAGGC126450.063.3211367
TATAGGG187700.063.204347
GTATAAG131100.063.130166
GGTATAC92800.062.9017335
TTATAGC117500.062.6637346
CTATAAC70150.062.507466
GGTATAA124950.062.4853065
Produced by FastQC (version 0.11.5)