FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS60mS3-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS60mS3-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247149
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG30500.065.747386
GGTATAG57100.065.397585
CGTATAG30800.064.994815
CTATACG20050.064.7564856
CGTATAA25900.064.589445
TTATAGG52200.064.495346
CCTATAC18950.064.4539645
GTATAGG60850.063.9540376
TATAGGC41200.063.7920347
GGTATAC30550.063.5792125
TATACGG34350.063.5742957
CTTATAG29300.063.3055275
GTATAGC36000.063.2852066
GGTATAA41300.062.9610755
GTTATAC31100.062.904955
TTATAGT57200.062.7733156
AGTATAG43250.062.6308365
TATAGTG68550.062.5392047
GTTATAG53600.062.4856835
CTATAAC19900.062.254966
Produced by FastQC (version 0.11.5)