FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS60mS3-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS60mS3-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8308324
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG92450.066.62376
CTATAGG122950.066.605546
CCTATAC74600.066.522855
CGTATAA103350.066.37115
TTATAGG202800.066.179046
CTTATAG106250.066.173555
GGTATAG217400.066.0825965
GTATAGG247150.065.2916266
TATACGG130200.064.939117
GCTATAC105400.064.8145755
CTTATAC101500.064.477625
AGTATAG159600.064.4466255
CGTATAG135800.064.376325
GGTATAC118650.064.360665
GTATAAG165500.064.008476
TTATAGT194850.063.9579546
GTTATAG211800.063.9137045
TATAGGG230500.063.6763157
CTATAAC81350.063.6688736
TATAGGT251050.063.538147
Produced by FastQC (version 0.11.5)