FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS60mS2-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS60mS2-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2636587
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG34600.065.841156
CCTATAC23300.065.483415
CGTATAG34900.065.0758745
CTTATAG34050.065.055995
TTATAGG60200.064.814316
CTATACG23450.064.7648546
GTATAGG66150.064.697746
GGTATAG64200.064.5382845
TATACGG38150.064.2090157
CGTATAA31900.064.175015
AGTATAG48550.063.429925
GGTATAC35550.063.2953955
TATAGGG65950.063.1429187
GTATAGC40700.062.6795166
TATAGGC49450.062.6281367
CTATAAC24550.062.433136
GTTATAA45750.062.187085
GCTATAC28600.062.035845
GGTATAA47950.061.9611975
GTATAGT66300.061.753966
Produced by FastQC (version 0.11.5)