FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS60mS2-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS60mS2-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7357653
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG183050.066.0555655
CTATAGG103050.065.917366
TTATAGG169100.065.418896
GTATAGG204650.065.358376
CGTATAA85950.065.352215
CCTATAC60750.065.2125555
CTATACG71650.065.0594256
CTTATAG92550.065.040395
CGTATAG111900.064.583435
CTATAAC70450.063.5844966
TATAGGG192900.063.5517047
GCTATAC88900.063.301715
CTTATAC87600.063.1224825
TATACGG107400.063.116757
TATAGGT196750.063.0729757
GTTATAG183350.062.9889145
GGTATAA133250.062.8501935
GGTATAC104300.062.8470045
AGTATAG137900.062.8372965
TTATAGT171550.062.8117946
Produced by FastQC (version 0.11.5)