FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS60mS1-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS60mS1-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4577776
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC34900.065.680265
CGTATAG54950.065.4066245
GTATAGG103850.065.3416756
CTATAGG59800.065.251826
GGTATAG102850.065.194385
CGTATAA54250.064.96045
TTATAGG95750.064.7655266
CTTATAG55200.064.413015
CTATACG43800.064.159426
GGTATAC56200.063.080065
TATAGGG106800.062.914247
TATAGGC73850.062.599567
TATACGG65700.062.534797
GCTATAC52900.062.450415
AGTATAG78350.062.4434855
GTTATAA74600.062.3924185
GTATAAG79900.062.1081246
GTATAGC67700.062.0830886
TTATAGC74700.061.980956
CTATAAC43550.061.9562576
Produced by FastQC (version 0.11.5)