FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS60mS1-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS60mS1-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7371728
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG190800.066.5828255
CTATACG85100.066.3316966
CCTATAC66050.065.9675755
CGTATAA92750.065.806165
CTATAGG107850.065.805796
TTATAGG173400.065.692866
GTATAGG211800.065.0677956
CGTATAG126600.064.990965
CTTATAG98600.064.9188465
TATACGG117700.064.7586447
GCTATAC96700.064.638165
TTATACG108850.064.558236
CCTATAG73250.064.165625
AGTATAG146650.064.028395
GTATAAG147350.063.9828766
GTTATAG180000.063.947765
TATAGGC143400.063.597727
CTATAAC73050.063.524266
CTTATAC94000.063.4790655
GTATAGC139900.063.462786
Produced by FastQC (version 0.11.5)