FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS5mS3-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS5mS3-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5363169
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG134800.065.161556
CTATAGG74250.065.041456
CTATACG51200.064.9323656
CGTATAG71100.064.823415
CCTATAC46100.064.677675
TTATAGG127700.064.646456
CTTATAG69100.064.0154345
GGTATAG129500.063.992285
CGTATAA63600.063.6637045
TATACGG77600.063.2708327
TATAGGG143650.063.144717
GGTATAC71000.062.9924055
GTATAGC82250.062.629586
GCTATAC58900.062.6240355
GTTATAA92050.062.578015
TATAGGC93150.062.4389727
TTATAAG93150.062.3638346
CTATAAC50000.062.221346
GGTATAA99100.062.1519435
TTATAGC88300.062.103476
Produced by FastQC (version 0.11.5)