FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS5mS3-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS5mS3-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8697946
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG207350.066.208856
CTATAGG129350.066.0123446
GGTATAG245600.065.9865955
CTATACG90900.065.6390466
CCTATAC72050.065.6191565
CGTATAA109550.065.326625
CTTATAG114600.065.257315
CCTATAG81950.064.353715
TATACGG139750.064.229917
GTTATAG227000.064.1631245
GTATAGG279250.064.1245046
TTATAGT215950.064.07456
CGTATAG154700.063.9279985
CTTATAC100050.063.5546155
GATATAG150350.063.4732865
TATAGTG295500.063.3811957
GGTATAC129300.063.1700975
GTATAGC163500.063.097416
TATAGGT265200.063.0484437
TATAGGC169100.062.953147
Produced by FastQC (version 0.11.5)