FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS5mS2-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS5mS2-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12024945
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG168350.065.854526
CTATACG116050.065.166316
CGTATAG163900.065.165715
TTATAGG278800.065.033096
GGTATAG284700.065.0129245
CCTATAC93200.064.997215
GTATAGG304850.064.917236
CGTATAA149050.064.591035
CTTATAG153750.064.551255
GTATAGC185100.063.65766
TATACGG180050.063.6540957
GGTATAC159000.063.2342035
TATAGGC213600.063.1420337
AGTATAG222450.063.0377165
TATAGGG309050.062.981127
GCTATAC136750.062.8001635
TTATAGC195100.062.565196
GGTATAA214250.062.4775735
CTATAAC113050.062.468956
TATAGTG312200.062.334457
Produced by FastQC (version 0.11.5)