FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS5mS2-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS5mS2-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7791845
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA95200.066.134945
TTATAGG179600.065.861316
CTATACG76950.065.853676
CTTATAG99850.065.648755
CTATAGG110900.065.5743566
GTATAGG206000.065.558286
GGTATAG188950.065.512615
CCTATAC66250.065.346245
CGTATAG115650.065.092585
GCTATAC94300.064.502255
GGTATAC113650.064.2056665
TATAGGG201300.064.09797
GTATAAG151950.063.7507676
AGTATAG144950.063.4276475
CTATAAC77000.063.356636
TTATAGT177800.063.300096
TATACGG116600.063.2983637
GTTATAG187800.063.1557245
TATAGGC142000.063.1400957
CTTATAC94750.063.0878035
Produced by FastQC (version 0.11.5)