FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS5mS1-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS5mS1-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15900357
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG216250.065.717656
CCTATAC120750.065.586365
TTATAGG369900.065.410926
CGTATAA195700.065.306455
CGTATAG212150.065.207855
GTATAGG402100.065.185796
CTTATAG204100.065.053465
CTATACG153950.064.807126
GGTATAG365850.064.749675
GGTATAC205350.064.163255
TATACGG227050.063.7016147
GTATAGC238400.063.413646
TATAGGG411100.063.2822077
AGTATAG293750.062.9745945
GCTATAC176050.062.914945
CTATAAC146850.062.8407486
TATAGGC283850.062.407937
TTATAGC259200.062.253726
TATAGTG417800.062.225517
TATAGGT414150.062.216237
Produced by FastQC (version 0.11.5)