FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS5mS1-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS5mS1-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9694449
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC82150.066.115985
CGTATAA121300.065.982335
CTATAGG141050.065.872256
CTATACG101200.065.668266
GGTATAG239150.065.5879065
CTTATAG124150.065.454245
TTATAGG231400.065.363086
GTATAGG267950.065.2498866
GGTATAC132950.064.675395
GCTATAC118350.064.433435
CGTATAG147050.064.399885
TATACGG152700.064.124217
AGTATAG189750.063.629665
TTATACG140600.063.5691876
TATAGGG253000.063.4070327
TATAGGC187650.063.2773937
TATAGTG274450.063.2203067
GTTATAG239550.063.155535
TTATAGT220800.063.0963486
GTATAGC163350.063.0281566
Produced by FastQC (version 0.11.5)