FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS45mS3-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS45mS3-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4115709
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG53250.065.0638966
CCTATAC31500.064.995855
CGTATAA48150.064.616855
CTATACG37450.064.292626
TTATAGG94300.063.8324136
GTATAGG100850.063.469096
GGTATAG95550.063.3292925
CGTATAG52400.063.3165665
CTTATAG51450.063.0571985
GGTATAC51950.063.0566065
TATACGG56600.062.5121547
GTATAGC61650.062.1592146
TATAGTG114700.062.0607877
AGTATAG74000.061.9555055
GCTATAC44450.061.885815
CTATAAC37000.061.7640766
CCCTATA33550.061.7538764
TATAGGG103950.061.6443987
TATAGGT101800.061.605577
CGCTATA39400.061.467414
Produced by FastQC (version 0.11.5)