FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS45mS3-N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS45mS3-N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7394789
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG104000.066.7962656
CTATACG81600.066.562036
CGTATAA93700.066.33375
GGTATAG179650.066.07845
CCTATAC63300.065.9580155
TTATAGG176050.065.878176
CTTATAG93300.065.230215
CGTATAG116850.064.9323965
AGTATAG139150.064.812995
GTATAGG196800.064.764926
GGTATAC105600.064.426315
TTATACG105400.064.2156456
TATACGG118600.064.1209647
TTATAGT170300.063.7458846
GCTATAC94850.063.684595
TATAGGG190550.063.6016737
GTATAGC121400.063.593376
CTTATAC91000.063.5330245
TATAGGC140050.063.4960257
GTATAAG146900.063.441646
Produced by FastQC (version 0.11.5)