FastQCFastQC Report
Sat 2 Nov 2019
HJ5LLBGXC_n01_MTS45mS2-noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5LLBGXC_n01_MTS45mS2-noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3757432
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC30650.065.537845
CTATAGG50800.065.4423756
CTATACG38150.064.3934566
TTATAGG88850.064.277936
GGTATAG84300.064.2204745
GTATAGG93800.064.01976
CTTATAG47800.063.4748885
CGTATAG49750.063.4489175
GGTATAC43550.063.0800825
GCTATAC42200.062.7760775
TATAGGG96900.062.6947147
CGTATAA44050.062.6818545
TATAGGC64300.062.0982867
TATACGG48650.062.0773167
GTATAGC52500.062.0568246
GTTATAG89500.062.0532655
CGCTATA40850.062.023644
TATAGGT95150.061.8617447
GGTATAA68500.061.765575
TATAGTG103150.061.54227
Produced by FastQC (version 0.11.5)